This textbook is the introductory manual for the students of biochemistry at an entry level to teach. The objective is to seed knowledge of basic biochemical principles and protocols and to increase the interest of the students in the subject matter. The target audiences are undergraduate chemistry, biochemistry, biomedical sciences, molecular biology, and biotechnology students. The present book is written in simple language covering the various aspects of basic biochemical parameters and a clear-cut practical concept has been offered in the in respect of all quantitative and qualitative experiments.