There are various text books, review and research articles dealing with various aspects of molecular biology of microorganisms. However, the reader usually gets disappointed in initiating an experiment due to complex protocols. In this concern, an assorted laboratory manual for molecular cloning not only designed to encourage the researchers and students, but also planned to give the scientific knowledge as well as the scientific aptitude. The proposed book on practical biotechnology comprises the practical procedures including aim, principle, material required, procedure and trouble shootings guides. This laboratory manual is presenting the protocols in a very simpler form for easy understanding of the undergraduates,postgraduates and researchers. Also the brief knowledge about principle and role of reagents used in the experiments will be useful for long run as well as it will be helpful to modify the protocol and reagents as per the their requirements and availability. Moreover, the trouble shooting guides will facilitate to avoid the negative result of the particular experiments.