A number of biochemistry manuals are commercially available that cover various experimental protocols of biochemistry but these manuals are usually away from the approach of the students due to their high cost. The purpose of this manual is to provide the authentic material to the students in affordable price. Very simple language is used in the manual so it is easy to understand. This manual covers the basic principle, reagents, equipment and protocols for various experiments/techniques commonly used in the biochemistry research. I hope this manual will be very helpful for young researchers. The contributing authors are acknowledged for sharing their knowledge and experiences by writing different chapters.