A Novel Cold Active Esterase from the Marine Psychrotolerant Isolate Pseudoalteromonas Arctica sp. nov. - Al Khudary, Rami
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In recent years, growing attention has been devoted to cold adapted microorganisms and their enzymes. A gene library was constructed using the genome of a new aerobic psychrotolerant marine isolate Pseudoalteromonas arctica sp. nov. The gene encoding an esterase was identified by screening the library on tributyrin plates. The recombinant protein was purified (3,3 - fold) in one step using a Ni- column and was subsequently characterised. The enzyme has unique properties since it requires a very low activation energy and retains 50% of its maximal activity at the freezing point of water.

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Produktbeschreibung
In recent years, growing attention has been devoted to cold adapted microorganisms and their enzymes. A gene library was constructed using the genome of a new aerobic psychrotolerant marine isolate Pseudoalteromonas arctica sp. nov. The gene encoding an esterase was identified by screening the library on tributyrin plates. The recombinant protein was purified (3,3 - fold) in one step using a Ni- column and was subsequently characterised. The enzyme has unique properties since it requires a very low activation energy and retains 50% of its maximal activity at the freezing point of water.
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Autorenporträt
Rami Al Khudary Born in Tripoli - Lebanon 19.11.1977 Educational background . October 2006: A PHD (Doctor rer. nat.) degree at the Technical University of Hamburg - Harburg (TUHH) "Institute of Technical Microbiology & Biotechnology" . June 2002: M.S. in Biology; Lebanese American University - Byblos (LAU) . July 1999: Teaching Diploma (for the Intermediate and Secondary classes); American University of Beirut (AUB) . July 1998: B.S. in Biology; American University of Beirut (AUB) . June 1995: Received the Lebanese Baccalaureate (II) at the Tripoli Evangelical School for Girls & Boys (TES) (Experimental Sciences Section) Projects and esearch . Worked on the screening, cloning, purification and characterization of esterases, lipases, phytases and pullulanases (TUHH). . Joined an expedition to the Azores Islands (Portugal) to collect volcanic water and sediment samples for the isolation and screening of extremophilic microorganisms and enzymes. (TUHH) . Biodegradation of Olive oil rich Wastewater under Aerobic & Mesophilic Conditions (LAU). . M.S. Thesis - Isolation and study of an extremely thermostable lipase of an olive oil degrading Thermoactinomyces sp.- (LAU) . Whey utilization for the production of Single cell proteins.(LAU)