Alfonso Olaya Abril

A pneumococcal protein glycocapture approach

to discover surface proteins by using the reference strain Streptococcus pneumoniae R6

• Broschiertes Buch

Produktbeschreibung

Emergence of pneumococcal multi-drug resistant clinical isolates prompts the need for a more effective vaccine for the prevention of disease. For this purpose, glycoproteins could be excellent candidates because of their implication in a number of biological processes, such as cell-to-cell recognition and the host immune response. In this study, methods widely used to capture potential surface proteins independently of their glycosylation status were carried out, and Concanavalin A (ConA) and Hydrazide subproteomes were obtained from different pneumococcal subcellular fractions (membrane, lysate, secretome and membrane-derived vesicles (MVs)) followed by protein LC-MS/MS analysis. In addition, a 2DE multilectinblotting (2DEMLB) analysis, using ConA, WGA, UEA, BC-1 and PNA lectins, was performed. Alfonso Olaya Abril , Lidia Gómez Gascón, Irene Jiménez Munguía, Michael J. McConnell, Meritxell García Quintanilla, Ignacio Obando Santaella, Manuel José Rodríguez Ortega.

Produktdetails

• Verlag: Editorial Académica Española
• Seitenzahl: 68
• Erscheinungstermin: 9. Februar 2018
• Spanisch
• Abmessung: 220mm x 150mm x 5mm
• Gewicht: 119g
• ISBN-13: 9786202101806
• ISBN-10: 6202101806
• Artikelnr.: 51398308

Autorenporträt

Doctor en Biomedicina por la Universidad de Córdoba (UCO), mi tesis se basó en la búsqueda de candidatos proteicos con fines vacunales y el desarrollo de herramientas de diagnóstico. Además de varias publicaciones científicas publicadas en revistas internacionales, el doctorado me permitió obtener el Premio Extraordinario de Doctorado de la UCO.