This lab manual is designed to give students experience with a wide variety of model systems currently in use by developmental biologists. Experiments range from classic slide or whole animal observations to more modern immunohistochemistry and manipulation of gene expression. All of these experiments can be completed on a relatively small budget.
This lab manual is designed to give students experience with a wide variety of model systems currently in use by developmental biologists. Experiments range from classic slide or whole animal observations to more modern immunohistochemistry and manipulation of gene expression. All of these experiments can be completed on a relatively small budget.Hinweis: Dieser Artikel kann nur an eine deutsche Lieferadresse ausgeliefert werden.
I am the daughter of a neuroscientist father and a biology-inclined mother. In large part due to the influence of my parents, I'd planned to be a marine biologist since my early teens. At UC Santa Cruz, I pursued a degree in Marine Biology and became very interested in sensory systems of deep sea fish. My master's degree work at Moss Landing Marine Labs gave me a chance to catch and examine deep sea fish sensory systems with the aim of finding out how they locate mates. My interest gradually changed from olfactory systems to vision in time for Ph.D. work on the central visual processing systems of goldfish. A post-doctoral fellowship at the Scripps Institution of Oceanography gave me the opportunity to study yet another fish sensory system; the development of the lateral line system in sturgeon. Following my stint in San Diego, I accepted a tailor-made position for a developmental-marine biologist at Stetson University. My current research focuses on spring fish ecology & population dynamics and the impact of common pollutants on amphibian development.
Inhaltsangabe
* Table of Contents * The Experiments * Introduction to Developmental Biology: Embryo Protocols, Ethics and Model Systems * Axial Patterning: Using Retinoic Acid to Disrupt Homeobox Gene Expression in Axolotls * Plant Cell Totipotency: Growing a Carrot from Adult Cells * Fertilization: Sea Urchin Syngamy and Development * Early Plant Development: Pollen Tube Formation * Morphogenesis: Creating Fate Maps of Albino Axolotls Using a Vital Dye * Cell Adhesion: Cell-Cell Interactions in Sponges and Amphibians * Embryogenesis: Chick and Amphibian Development (Slide-based) * Neurulation: Labelling Chick Notochord using Immunocytochemistry * Gametogenesis: Comparison of Plants and Animals * Regeneration: Morphallaxis and Epimorphosis * Metamorphosis: Drosphila Imaginal Discs * External Influences on Development: Design Your Own Experiment with Amphibians, Chicks or Fish * Information for the Instructor * Animal Care and Maintenance * Animal and Plant Sources * Chemical Sources * Slides Needed / Sources * Solutions and Other Materials * Glossary * Literature Cited
* Table of Contents * The Experiments * Introduction to Developmental Biology: Embryo Protocols, Ethics and Model Systems * Axial Patterning: Using Retinoic Acid to Disrupt Homeobox Gene Expression in Axolotls * Plant Cell Totipotency: Growing a Carrot from Adult Cells * Fertilization: Sea Urchin Syngamy and Development * Early Plant Development: Pollen Tube Formation * Morphogenesis: Creating Fate Maps of Albino Axolotls Using a Vital Dye * Cell Adhesion: Cell-Cell Interactions in Sponges and Amphibians * Embryogenesis: Chick and Amphibian Development (Slide-based) * Neurulation: Labelling Chick Notochord using Immunocytochemistry * Gametogenesis: Comparison of Plants and Animals * Regeneration: Morphallaxis and Epimorphosis * Metamorphosis: Drosphila Imaginal Discs * External Influences on Development: Design Your Own Experiment with Amphibians, Chicks or Fish * Information for the Instructor * Animal Care and Maintenance * Animal and Plant Sources * Chemical Sources * Slides Needed / Sources * Solutions and Other Materials * Glossary * Literature Cited
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