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It gives me immense pleasure to write "A Practical Manual for Basic Techniques in Molecular Biology", for the students and researchers in the area of molecular biology. Molecular Biology and Biotechnology is a multidisciplinary field, now it is a mandatory course in most colleges, universities and research labs. This manual is useful for learner students, for setting up laboratory experiments and as a teaching aid. This practical manual is a collection of basic and applied methodologies in the arena of Molecular Biology, which can be used in different fields of biology. This book is compiled by considering the practical experiments that can be extensively used in educational courses and research laboratories. In this book, protocols are distributed in different chapters for understanding the purpose. As compared to other teaching practical manuals, this is having detailed steps for proper execution. This information will certainly allow students to plan, execute and make modifications to the method(s) of their interest.