Breast and cervical cancers are the most common type of cancer among the female population of the Western world and is the leading cause of death in women. This disease can be cure if detected as early as possible. In Chapter 1, of the various conventional techniques in use, a brief discussion on how a healthy cervical tissue progresses into a cancerous one is discussed. Chapter 2 gives the description of Instrumentation involved in the photoluminescence spectroscopy and also the measurement of Polarized Emission of Fluorescence. Chapter 3 gives the theoretical background on Fluorescence spectroscopy and the methodology to extract Intrinsic Fluorescence. Chapter 4 and 5 compares the extracted intrinsic and bulk Fluorescence from the various Fluorophores found in normal and cancerous cervical and breast tissues. Chapter 6 is the conclusion of the research carried out on normal and cancerous cervical and breast tissues and discussion of the result obtained. I hope that the book will fulfil the need of reader. I shall greatly appreciate the critics and valuable suggestions for the improvement of quality and content of the book, from the readers at iitk.ejaz@gmail.com.