In this book, an experimental study has been described i. e., 1. Media for Biochemical Test, 2. Cultivation Media, 3. Staining Methods, 4. Reagents, 5. The Standard Solutions. In addition to these, Alcaligenes medium, Balch, Algal medium, Basal medium, Alternasia medium, Basdiomycets medium. Carbon utilization medium, Eijkman text, Carry-Blair medium, Eimhjellen medium, Cell wall stairling, Erwinia selective medium, Chaetomium medium, Chitin hydrolyses, 3 Esculin hydrolysis, Hugh and Leifson medium, Litmus milk medium, Hungate, Litmus milk test, India ink staining method, Mandel and Reese medium, Indole test, Media for biochemical test, Nitrate reduction, Reagents, Nitrification test, buffers, Nitrosomonasm edium, 108 fixatives, Noren's medium, IMVIC test, Normality, Oatmeal agar, Rhizobium medium, ONPG test, Rhizoctonia agar and manymore.