This research work was done to know the effect of abdominal insufflation and study the effect of antibiotics on bacterial growth; especially in cases of peritonitis. There were few schools of thoughts regarding use of abdominal insufflation during laparoscopy and gasless laparoscopy in cases of peritonitis. Few studies suggested that gasless laparoscopy is better, in other studies it was seen that there is no difference in abdominal insufflation and gasless laparoscopy. The attendant mortality and morbidity was more in some studies when laparoscopy was done using abdominal insufflation. By this work we tried to see the effect of abdominal insufflation on bacterial growth in abdominal insufflation, non insufflation and effect of antibiotics on bacterial growth in experimental model of rats. This book is targeted at the young researchers having innovative concepts in surgical field.