Activation tagging in transgenic poplar - Sherif, Fadia El;Kamal, Hamdy;Fladung, Matthias
49,00 €
inkl. MwSt.
Versandkostenfrei*
Versandfertig in 6-10 Tagen
  • Broschiertes Buch

We describe the establishment of an activation tagging system in poplar based upon the maize Ac/Ds transposable element system. Transgenic poplar plants were obtained carrying either one or two of the above mentioned constructs Following induction of the GIP promoter only a weak transposase signal could be detected in RT- PCR experiments. Mobility of the ATDs activation construct could not be confirmed in the GIP system. An Activation Tagging experiment was initiated using eight different double transgenic lines. A few putative variants could be obtained revealing deficiency in chlorophyll…mehr

Andere Kunden interessierten sich auch für
Produktbeschreibung
We describe the establishment of an activation tagging system in poplar based upon the maize Ac/Ds transposable element system. Transgenic poplar plants were obtained carrying either one or two of the above mentioned constructs Following induction of the GIP promoter only a weak transposase signal could be detected in RT- PCR experiments. Mobility of the ATDs activation construct could not be confirmed in the GIP system. An Activation Tagging experiment was initiated using eight different double transgenic lines. A few putative variants could be obtained revealing deficiency in chlorophyll content. Single transgenic aspen lines carrying HSP- transposase have been produced. Following transformation with HSP-transposase a chimerical transgenic line was obtained revealing white and green plants. Following activation tagging experiment a HSP-transposase/ATDs-rolC double transgenic line, one somaclonal variation line was obtained revealing white plants.