Activity-Based Proteomics

Methods and Protocols
Herausgegeben:Overkleeft, Herman S.; Florea, Bogdan I.

• Gebundenes Buch

Produktbeschreibung

This volume focuses on explorative activity-based proteomics, biomedical applications of activity-based proteomics, and chemical strategies in activity-based proteomics providing a concise overview of activity-based protein profiling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Activity-Based Proteomics: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Produktdetails

• Methods in Molecular Biology 1491
• Verlag: Humana / Springer New York / Springer, Berlin
• Artikelnr. des Verlages: 978-1-4939-6437-6
• 1st ed. 2017
• Seitenzahl: 236
• Erscheinungstermin: 25. Oktober 2016
• Englisch
• Abmessung: 260mm x 183mm x 18mm
• Gewicht: 631g
• ISBN-13: 9781493964376
• ISBN-10: 1493964372
• Artikelnr.: 45069472

Inhaltsangabe

Activity-Based Protein Profiling - From Chemical Novelty to Biomedical Stalwart.- A Quantitative Mass-Spectrometry Platform to Monitor Changes in Cysteine Reactivity.- Activity-Based Protein Profiling with Natural Product-Derived Chemical Probes in Human Cell Lysates.- Inhibitor Discovery by Convolution Activity-Based Protein Profiling.- Activity-Based Protein Profiling in Bacteria.- Activity-Based Lipid Esterase Profiling of M. Bovis BCG at Different Metabolic States Using Tetrahydrolipstatin (THL) as Bait.- Comparative Activity-Based Flavin-Dependent Oxidase Profiling.- Activity-Dependent Photoaffinity Labelling of Metalloproteases.- Profiling the Activity of Deubiquitinating Enzymes using Chemically Synthesized Ubiquitin-Based Probes.- Profiling Protein Sumoylation using Biochemical and Mass Spectrometry-Based Research Tools.- Live Cell Imaging and Profiling of Cysteine Cathepsin Activity using a Quenched Activity-Based Probe.- Competitive Activity-Based Protein Profiling Of Serine Hydrolases: A Case Study on DAGL-Alpha.- A Protocol for Protein Profiling using Chemoselective Cleavable Linker Probes in Semi-Permeabilized Cells.- Cleavable Linkers in Chemical Proteomics Applications.- Two-Step Activity-Based Protein Profiling With the Proteasome System as Model of Study.