Gebundenes Buch

Advanced Mammalian Cell Culture Techniques

Principles and Practices

Herausgegeben: Ramkumar, K.M.; Senthilkumar, R.; Hoque, Md Enamul
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This up-to-date book compiles both basic and advanced laboratory techniques of mammalian cell culture. It is divided into four major sections encompassing the basics of cell culture, nucleic acid and protein isolation, cell-staining techniques, and cell transfection and single-cell analysis. The topics include aseptic handling, media preparation, and passaging of cells. The book also outlines downstream assays such as nucleic acid and protein isolation from in-vitro cell cultures.Key Features:Covers cellular staining using fluorescent dyes, genetic manipulation of cells via transfection, and a...