I. Cell-Cell Communication and Cell Extracellular Matrix Interactions.- Extracellular Matrix Cell Differentiation.- Structure and Function of the Heparan Sulfate Proteoglycans.- Role Played by Gap Junctions in Cell-Cell Communication.- Fibronectin-Mediated Cellular (Inter)actions.- II. "Normal" Versus "Abnormal" Cells.- Use of Cell Lines in Biotechnology.- Why Not Use Primary Cells.- III. Large Scale Cultivation of Animal Cells.- Technology of Mass Cultivation: An Overview.- Cell Culture in Hollow Fibre Bioreactors.- Perfusion Culture.- Good Manufacturing Practice in the Production of Biologicals by Cell Cultures.- Biosensors.- IV. Analysis of the Cell's Behaviour.- Effects of Retinoids on Growth Differentiation.- Phosphoinositide Metabolism and Control of Cell Proliferation.- Somatic Cell Genetic Analysis of Growth Control.- Promoter Organization of Eukaryotic Protein-Coding Genes.- V. Modification of the Cell Genome.- Tailoring of an Anti-Human Placental Alkaline Phosphatase Immunoglobulin Using Genetic Engineering.- Vaccinia Virus as an Expression Vector.- Expression and Integration of Exogenous DNA Seguences Transfected into Mammalian Cells.- Cooperation of Oncogenes in Malignant Transformation.- VI. Applications of Animal Cell Cultures.- Molecular Dissection of the Cell Cycle.- Use of Microbeads for Cell Transplantation.- Influence of Epigenetic Factors on Epidermal Differentiation and Morphogenesis.- The Characterization of Fibroblast Growth Factor and Its Biological Effect In Vitro and In Vivo.- Culture of Hybridomas - A Survey.- Applications of Chromatography in Downstream Processing.- General discussion.
I. Cell-Cell Communication and Cell Extracellular Matrix Interactions.- Extracellular Matrix Cell Differentiation.- Structure and Function of the Heparan Sulfate Proteoglycans.- Role Played by Gap Junctions in Cell-Cell Communication.- Fibronectin-Mediated Cellular (Inter)actions.- II. "Normal" Versus "Abnormal" Cells.- Use of Cell Lines in Biotechnology.- Why Not Use Primary Cells.- III. Large Scale Cultivation of Animal Cells.- Technology of Mass Cultivation: An Overview.- Cell Culture in Hollow Fibre Bioreactors.- Perfusion Culture.- Good Manufacturing Practice in the Production of Biologicals by Cell Cultures.- Biosensors.- IV. Analysis of the Cell's Behaviour.- Effects of Retinoids on Growth Differentiation.- Phosphoinositide Metabolism and Control of Cell Proliferation.- Somatic Cell Genetic Analysis of Growth Control.- Promoter Organization of Eukaryotic Protein-Coding Genes.- V. Modification of the Cell Genome.- Tailoring of an Anti-Human Placental Alkaline Phosphatase Immunoglobulin Using Genetic Engineering.- Vaccinia Virus as an Expression Vector.- Expression and Integration of Exogenous DNA Seguences Transfected into Mammalian Cells.- Cooperation of Oncogenes in Malignant Transformation.- VI. Applications of Animal Cell Cultures.- Molecular Dissection of the Cell Cycle.- Use of Microbeads for Cell Transplantation.- Influence of Epigenetic Factors on Epidermal Differentiation and Morphogenesis.- The Characterization of Fibroblast Growth Factor and Its Biological Effect In Vitro and In Vivo.- Culture of Hybridomas - A Survey.- Applications of Chromatography in Downstream Processing.- General discussion.
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