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This book collects the peer-reviewed contributions accepted for the publication in the Special Issue "Advances in In Situ Biological and Chemical Groundwater Treatment" of the MDPI journal Water. As such, the contributions refer to a variety of widespread pollutants (chlorinated ethenes, chlorinated phenols, chromium, copper, nickel, and arsenic phenols) and new remediation approaches (bioremediation, bioelectrochemical systems, and sorption), covering lab and field studies.