This book contain valuable information regarding microbial enzyme production with special reference to alpha amylase. This book is very useful to researchers working on research in field of microbial enzyme production and bioprocess engineering. Amylases constitute a class of industrial enzymes having approximately 20% of the enzyme market. -amylase has found its application in a range of industries including food, brewing, distilling industry, textile, paper pharmaceutical and bioconversion of solid waste etc. Microorganism used in present investigation, isolated from rhizospheric regions. A total of 30 natural isolates were scanned for -amylase activity out of which 20 isolates were biochemically characterized. Based on ribotyping all the isolates were identified and one of the most promising amylase producer i.e. Bacillus licheniformis was finally selected for further studies. Bacillus species produce the amylase because of its heterotrophic nature as it can grow on variety ofsubstrates ranging from pure compounds to solid wastes.