Alpha-Synuclein

Methods and Protocols
Herausgegeben:Bartels, Tim

• Gebundenes Buch

Produktbeschreibung

This volume provides the reader with detailed protocols that describe a variety of in vitro and in vivo techniques that study reductionist systems and human samples. The methods covered in this book explore a broad selection of topics such as functional aspects of alpha-synuclein biology, its lipid-interactions and misfolding, and functional deficits in cells and mice. Chapters also cover topics such as kinetic measurements of endocytosis and exocytosis in cultured neurons; electrochemiluminescence-based detection; yeast-based screens to target alpha-synuclein toxicity; mass-spectrometry of alpha-synuclein in rat cortical neurons; and expression and purification of untagged a-synuclein. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Alpha-Synuclein: Methods and Protocols is a valuable reference guide that aids researchers working on topics related to alpha-synuclein and its various aspects.

Produktdetails

• Methods in Molecular Biology 1948
• Verlag: Humana / Springer New York / Springer, Berlin
• Artikelnr. des Verlages: 978-1-4939-9123-5
• 1st ed. 2019
• Seitenzahl: 304
• Erscheinungstermin: 18. Februar 2019
• Englisch
• Abmessung: 260mm x 183mm x 22mm
• Gewicht: 748g
• ISBN-13: 9781493991235
• ISBN-10: 149399123X
• Artikelnr.: 54507415

Inhaltsangabe

Assays for Neuronal Defects Caused by Early Formation of -Synuclein Inclusions in Primary Cultured Neurons.- Immunohistochemical Detection of Alpha-Synuclein in Unfixed Human Brain Tissue.- Kinetic Measurements of Endocytosis and Exocytosis in Cultured Neurons.- Detection of Misfolded -Synuclein Aggregates in Cerebrospinal Fluid by the Protein Misfolding Cyclic Amplification Platform.- Stereotaxic Targeting of Alpha-Synuclein Pathology in Mouse Brain using Pre-Formed Fibrils.- Quantification of Alpha-Synuclein in Biological Fluids by Electrochemiluminescence-Based Detection.- Alpha-Synuclein Proximity Ligation Assay (AS-PLA) in Brain Sections to Probe for Alpha-Synuclein Oligomers.- Studying -Synuclein Conformation by Intact-Cell Crosslinking.- C. elegans as a Model for Synucleinopathies and Other Neurodegenerative Diseases: Tools and Techniques.- Recombinant Expression and Purification of N-Acetylated Alpha-Synuclein.- CircularDichroism and Isothermal Titration Calorimetry to Study the Interaction of -Synuclein with Membranes.- Yeast-Based Screens to Target Alpha-Synuclein Toxicity.- Probing Structural Changes in Alpha Synuclein by Nuclear Magnetic Resonance Spectroscopy.- Measuring Mitochondrial Dysfunction Caused by Soluble -Synuclein Oligomers.- Monitoring -Synuclein Proteotoxicity in Drosophila Models.- Detection of -Synuclein in Biological Samples using Mass Spectrometry.- Single-Molecule FRET Detection of Early-Stage Conformations in -Synuclein Aggregation.- In Situ Peroxidase Labelling and Mass-Spectrometry of Alpha-Synuclein in Rat Cortical Neurons.- Probing Alpha-Synuclein Conformations by Electron Paramagnetic Resonance (EPR) Spectroscopy.- Expression and Purification of Untagged -Synuclein.- Generation and Analysis of Viral Vector Mediated Rodent Models for Parkinson's Disease.