The combined evaluation of physiology and behaviour allows for a complete and comprehensive pre-clinical assessment of central nervous system (CNS) functions both in physiological and pathological conditions. An integrated video-telemetric electroencephalography (Video-tEEG) system was developed to enable simultaneous assessment of animal behaviour by video tracking technologies, and electroencephalographic analysis by radio-telemetry in freely moving rats. This integrated collection of EEG waveforms and activities of animals provides useful and powerful insights into the correlations between behavioral and electrical brain s activities in different and important areas of research (e.g., convulsion risk assessment, drug-induced sleep-wakefulness disruption, environmental conditions and animal welfare evaluations), also allowing for a reduction of the number of animals used, improving and refining the data quality by maximizing the amount of information gained from each experimental research.