A completely revised edition of a comprehensive and popular introduction to the fast moving area of forensic genetics. The text begins with key concepts needed to fully appreciate the subject and moves on to examine the latest developments in the field. Illustrated in full colour throughout, this accessible textbook includes numerous references to relevant casework. With information on the full process of DNA evidence from collection at the scene of a crime to presentation in a legal context this book provides a complete overview of the field. Key Features: Greater inÂ-depth coverage of…mehr
A completely revised edition of a comprehensive and popular introduction to the fast moving area of forensic genetics. The text begins with key concepts needed to fully appreciate the subject and moves on to examine the latest developments in the field. Illustrated in full colour throughout, this accessible textbook includes numerous references to relevant casework. With information on the full process of DNA evidence from collection at the scene of a crime to presentation in a legal context this book provides a complete overview of the field. Key Features: Greater inÂ-depth coverage of sequencing technology; updates and expansion of collection methods, characterization of body fluids using RNA, transfer of DNA; significant updates next generation sequencing, rapidly mutating STRs, whole mtDNA sequencing, X chromosome markers. New chapter on phenotype prediction and prediction of biological ancestry Self-assessment questions to aid student understanding throughout the text. Companion website
Dr Will Goodwin, Senior Lecturer in Forensic Genetics, Faculty of Science and Technology, University of Central Lancashire. Dr Sibte Hadi, Senior Lecturer in Forensic Genetics, Faculty of Science and Technology, University of Central Lancashire. Dr Adrian M. T. Linacre, Department of Pure and Applied Chemistry, University of Strathclyde.
Inhaltsangabe
Foreword. Preface. 1 Introduction to forensic genetics. Forensic genetics. A brief history of forensic genetics. 2 DNA structure and the genome. DNA structure. Organization of DNA into chromosomes. The structure of the human genome. Genetic diversity of modern humans. The genome and forensic genetics. Tandem repeats. Single nucleotide polymorphisms. 3 Biological material-collection, characterization and storage. Sources of biological evidence. Collection and handling of material at the crime scene. Identification and characterization of biological evidence. Evidence collection. Sexual and physical assault. Storage of biological material. 4 DNA extraction and quantification. DNAextraction. General principles of DNA extraction. DNA extraction from challenging samples. Quantification of DNA. DNA IQ system. 5 Polymerase chain reaction. The evolution of PCR-based profiling in forensic genetics. DNA replication: the basis of the PCR. The components of PCR. Taq DNA polymerase. The PCR process. PCR inhibition. Sensitivity and contamination. The PCR laboratory. 6 The analysis of short tandem repeats. Structure of STR loci. The development of STR multiplexes. Detection of STR polymorphisms. Interpretation of STR profiles. 7 Assessment of STR profiles. Stutter peaks. Split peaks (±N). Pull-up. Template DNA Overloaded profiles. Low template DNA typing. Peak balance. Mixtures. Degraded DNA. PCR inhibition. 8 Statistical interpretation of STR profiles. Population genetics. Deviation from the Hardy-Weinberg equilibrium. Statistical tests to determine deviation from the Hardy-Weinberg equilibrium. Estimating the frequencies of STR profiles. Corrections to allele frequency databases. Which population frequency database should be used? Conclusions. 9 Evaluation and presentation of DNA evidence. Hierarchies of propositions. Likelihood ratios. Two fallacies. Comparison of three approaches. 10 Databases of DNA profiles. The UK National DNA Database. Legislation in England and Wales. International situation. 11 Kinship testing. Parentage testing. Punnett square. Identification of human remains. 12 Single nucleotide polymorphisms. SNPs-occurrence and structure. Detection of SNPs. SNP detection for forensic applications. Forensic applications of SNPs. SNPs compared with STR loci. 13 Lineage markers. Mitochondria. Applications of mtDNA profiling. Haplotypes and haplogroups. The Y chromosome. Forensic applications of Y chromosome polymorphisms. 14 Non-human DNA typing. Non-human sample types. Species identification. Linkage to an individual using STR Loci. Linkage to an individual using mitochondrial loci. Microbial DNA testing. Concluding comments. Appendix A: Forensic parameters. Appendix B: Useful web links. Glossary. Abbreviations. Index.
Foreword. Preface. 1 Introduction to forensic genetics. Forensic genetics. A brief history of forensic genetics. 2 DNA structure and the genome. DNA structure. Organization of DNA into chromosomes. The structure of the human genome. Genetic diversity of modern humans. The genome and forensic genetics. Tandem repeats. Single nucleotide polymorphisms. 3 Biological material-collection, characterization and storage. Sources of biological evidence. Collection and handling of material at the crime scene. Identification and characterization of biological evidence. Evidence collection. Sexual and physical assault. Storage of biological material. 4 DNA extraction and quantification. DNAextraction. General principles of DNA extraction. DNA extraction from challenging samples. Quantification of DNA. DNA IQ system. 5 Polymerase chain reaction. The evolution of PCR-based profiling in forensic genetics. DNA replication: the basis of the PCR. The components of PCR. Taq DNA polymerase. The PCR process. PCR inhibition. Sensitivity and contamination. The PCR laboratory. 6 The analysis of short tandem repeats. Structure of STR loci. The development of STR multiplexes. Detection of STR polymorphisms. Interpretation of STR profiles. 7 Assessment of STR profiles. Stutter peaks. Split peaks (±N). Pull-up. Template DNA Overloaded profiles. Low template DNA typing. Peak balance. Mixtures. Degraded DNA. PCR inhibition. 8 Statistical interpretation of STR profiles. Population genetics. Deviation from the Hardy-Weinberg equilibrium. Statistical tests to determine deviation from the Hardy-Weinberg equilibrium. Estimating the frequencies of STR profiles. Corrections to allele frequency databases. Which population frequency database should be used? Conclusions. 9 Evaluation and presentation of DNA evidence. Hierarchies of propositions. Likelihood ratios. Two fallacies. Comparison of three approaches. 10 Databases of DNA profiles. The UK National DNA Database. Legislation in England and Wales. International situation. 11 Kinship testing. Parentage testing. Punnett square. Identification of human remains. 12 Single nucleotide polymorphisms. SNPs-occurrence and structure. Detection of SNPs. SNP detection for forensic applications. Forensic applications of SNPs. SNPs compared with STR loci. 13 Lineage markers. Mitochondria. Applications of mtDNA profiling. Haplotypes and haplogroups. The Y chromosome. Forensic applications of Y chromosome polymorphisms. 14 Non-human DNA typing. Non-human sample types. Species identification. Linkage to an individual using STR Loci. Linkage to an individual using mitochondrial loci. Microbial DNA testing. Concluding comments. Appendix A: Forensic parameters. Appendix B: Useful web links. Glossary. Abbreviations. Index.
Rezensionen
"William Goodwin, Sibte Hadi (both U. of Central Lancashire, England), and Adrian Linacre (Flinders U. Australia) provide a textbook for a first undergraduate course, covering all aspects of forensic genetics briefly, with references to more detailed coverage of particular aspects elsewhere." (Booknews, 1 April 2011)
Es gelten unsere Allgemeinen Geschäftsbedingungen: www.buecher.de/agb
Impressum
www.buecher.de ist ein Shop der buecher.de GmbH & Co. KG Bürgermeister-Wegele-Str. 12, 86167 Augsburg Amtsgericht Augsburg HRA 13309
