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It is known, according to recent research that there are morphological and physiological differences between cattle and buffaloes. Buffaloes have still semi-wild characteristics compared with other farm animals. Veterinarians always complain about the behavior and temperament of buffaloes which are vicious and indocile. In this trial, a comparative study of the recent well-known tranquillisers and sedatives was carried out to investigate their efficiency in overcoming the excitement and anxiousness usually manifested by such animals and in aiding veterinarians to deal safely with such species.