Benzodiazepines are used widely for treatment of central nervous system (CNS) disorders and there is a great need to review the analytical work reported so far in the literature. Numerous analytical procedures including chromatographic, spectrometric and electro analytical techniques were reported for the analysis of benzodiazepines in bulk drugs, pharmaceutical formulations and biological fluids. This book provides a brief overview of an assortment of validated analytical methods for the analysis of benzodiazepines. This is intended to help in selecting the analytical procedure for the analysis of benzodiazepines with great accuracy, precision and to develop more selective, sensitive, robustic method.