Analysis of Ribosomal Inactivating Proteins from Bougainville sps. - Mookavelar Pasupathy, Karthikeyan;Sundararaman, Geethalakshmi;Arumugam, Anitha
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In this study, RIPs were isolated from the root of Bougainvillea spectabilis and its action on the rRNA of E. coli and S. cerevisiea was determined. The Protein was purified by CM Sephadex Column Chromatography and quantified by Braford's method and its electrophoretic mobility and their molecular weight were determined by SDS PAGE and Native gel. Key words : Bougainvillea spectabilis , E. coli, S. cerevisiea , CM Sephadex Column Chromatography , Braford's method , SDS PAGE and Native gel. Our world is built on biology and once we begin to understand it, it then becomes a technology.

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Produktbeschreibung
In this study, RIPs were isolated from the root of Bougainvillea spectabilis and its action on the rRNA of E. coli and S. cerevisiea was determined. The Protein was purified by CM Sephadex Column Chromatography and quantified by Braford's method and its electrophoretic mobility and their molecular weight were determined by SDS PAGE and Native gel. Key words : Bougainvillea spectabilis , E. coli, S. cerevisiea , CM Sephadex Column Chromatography , Braford's method , SDS PAGE and Native gel. Our world is built on biology and once we begin to understand it, it then becomes a technology.
Autorenporträt
Karthikeyan M.P.Working as a Assistant Professor in the department ofBiotechnology, Nehru Arts And Science college, Coimbatore with 13 years of Teaching experience. Permanent Membership in 5 government affiliated organization. Editorial board Member in 3 international journals. Member in Bharathiar university M.Phil Board of studies.