The basis for this volume is the 11th Symposium on Analytical Ultracentrifugation held in March 25-26, 1999 at the University of Potsdam, Germany. This book presents a comprehensive collection of 33 contributions from leading scientists in this field including: Technical and methodological innovations.- Innovations in data analysis.- Hydrodynamics/Modelling.- Synthetic polymers, colloids and supramolecular systems.- Biological systems.- Interacting systems and assemblies. In contrast to the increasing significance of analytical ultracentrifugation, related modern books are very rare.…mehr
The basis for this volume is the 11th Symposium on Analytical Ultracentrifugation held in March 25-26, 1999 at the University of Potsdam, Germany. This book presents a comprehensive collection of 33 contributions from leading scientists in this field including: Technical and methodological innovations.- Innovations in data analysis.- Hydrodynamics/Modelling.- Synthetic polymers, colloids and supramolecular systems.- Biological systems.- Interacting systems and assemblies. In contrast to the increasing significance of analytical ultracentrifugation, related modern books are very rare. Therefore, this volume will be a helpful source of information to anyone who wants to catch up with the most recent developments and results related to this important analytical method.
Technical and methodological innovations.- The installation of an eight-cell Schlieren optics multiplexer in a Beckman Optima XLI/XL analytical ultracentrifuge used to measure steep refractive index gradients.- New facilities to improve the properties of Schlieren optics.- Shift interferometry in analytical ultracentrifugation of polymer solutions.- Investigation of the efficiencies of stabilizers for nanoparticles by synthetic boundary crystallization ultracentrifugation.- Innovations in data analysis.- A comparison of evaluation methods for sedimentation velocity data of polymer solutions.- Determination of the particle size distribution of 5-100-nm nanoparticles with the analytical ultracentrifuge: consideration and correction of diffusion effects.- Pseudo synthetic boundary experiments: a new approach to the determination of diffusion coefficients from sedimentation velocity experiments.- Application of the solvent density variation method to sedimentation velocity experiments on biological systems.- Hydrostatic pressure in ultracentrifugation.- The correct analysis of low-speed sedimentation equilibrium distributions recorded by the Rayleigh interference optical system in a Beckman XL-I ultracentrifuge.- Allowance for thermodynamic nonideality in the analysis of sedimentation equilibrium distributions reflecting complex formation between dissimilar reactants.- Hydrodynamics/Modeling.- Normalized scaling relations as a natural classification of linear macromolecules according to size.- Universal size-independent quantities for the conformational characterization of rigid and flexible macromolecules.- Calculation of structural parameters from hydrodynamic data.- Prediction of hydrodynamic parameters from 3D structures.- Synthetic polymers, colloids andsupramolecular systems.- Solution properties of supramolecular cobalt coordination arrays.- Working with multidetection in the analytical ultracentrifuge: the benefits of the combination of a refractive index detector and an absorption detector for the analysis of colloidal systems.- Sedimentation velocity analysis of oligomeric enzymes in hydrated reversed micelles of surfactants in organic solvents.- Spectroscopic and hydrodynamic investigations of nonionic and zwitterionic detergents.- Microemulsion polymerization of butyl acrylate and methyl methacrylate.- Sedimentation, translational diffusion, and viscosity of lactosylated polyamidoamine dendrimers.- Biological systems.- A conformation spectrum analysis of the morphological states of myosin S1 in the presence of effectors.- Combining ultracentrifugation with fluorescence to follow the unfolding of modules 16-17 of complement receptor type 1.- Determination of partial molal volumes, and salt and water binding, of highly charged biological macromolecules (tRNA, halophilic protein) in multimolar salt solutions.- Studying membrane proteins in detergent solution by analytical ultracentrifugation: different methods for density matching.- Analytical ultracentrifugation of the nitrogenase of Azotobacter vinelandii under anaerobic conditions.- Combining sedimentation velocity with SEC-MALLS to probe the molecular structure of heterogeneous macromolecular systems that cannot be preparatively separated: application to three rice starches.- Inhomogeneity and conformational parameters of low-substituted carboxymethyl cellulose from analytical ultracentrifugation data.- UV tagging leaves the structural integrity of an arabino-(4-O-methylglucurono)xylan polysaccharide unaffected.- Elevated-temperature analytical ultracentrifugation of a low-methoxy polyuronide.- Interacting systems and assemblies.- Towards deconvoluting the interaction of the Bacillus subtilis sporulation proteins SinR and SinI using tryptophan analogue incorporated proteins.- Use of the tryptophan analogue 7-azatryptophan to study the interaction of ? N with Escherichia coli RNA polymerase core enzyme.- Nonequilibrium self-association of a cpn60 chaperonin induced by tryptophan mutation.
Technical and methodological innovations.- The installation of an eight-cell Schlieren optics multiplexer in a Beckman Optima XLI/XL analytical ultracentrifuge used to measure steep refractive index gradients.- New facilities to improve the properties of Schlieren optics.- Shift interferometry in analytical ultracentrifugation of polymer solutions.- Investigation of the efficiencies of stabilizers for nanoparticles by synthetic boundary crystallization ultracentrifugation.- Innovations in data analysis.- A comparison of evaluation methods for sedimentation velocity data of polymer solutions.- Determination of the particle size distribution of 5-100-nm nanoparticles with the analytical ultracentrifuge: consideration and correction of diffusion effects.- Pseudo synthetic boundary experiments: a new approach to the determination of diffusion coefficients from sedimentation velocity experiments.- Application of the solvent density variation method to sedimentation velocity experiments on biological systems.- Hydrostatic pressure in ultracentrifugation.- The correct analysis of low-speed sedimentation equilibrium distributions recorded by the Rayleigh interference optical system in a Beckman XL-I ultracentrifuge.- Allowance for thermodynamic nonideality in the analysis of sedimentation equilibrium distributions reflecting complex formation between dissimilar reactants.- Hydrodynamics/Modeling.- Normalized scaling relations as a natural classification of linear macromolecules according to size.- Universal size-independent quantities for the conformational characterization of rigid and flexible macromolecules.- Calculation of structural parameters from hydrodynamic data.- Prediction of hydrodynamic parameters from 3D structures.- Synthetic polymers, colloids andsupramolecular systems.- Solution properties of supramolecular cobalt coordination arrays.- Working with multidetection in the analytical ultracentrifuge: the benefits of the combination of a refractive index detector and an absorption detector for the analysis of colloidal systems.- Sedimentation velocity analysis of oligomeric enzymes in hydrated reversed micelles of surfactants in organic solvents.- Spectroscopic and hydrodynamic investigations of nonionic and zwitterionic detergents.- Microemulsion polymerization of butyl acrylate and methyl methacrylate.- Sedimentation, translational diffusion, and viscosity of lactosylated polyamidoamine dendrimers.- Biological systems.- A conformation spectrum analysis of the morphological states of myosin S1 in the presence of effectors.- Combining ultracentrifugation with fluorescence to follow the unfolding of modules 16-17 of complement receptor type 1.- Determination of partial molal volumes, and salt and water binding, of highly charged biological macromolecules (tRNA, halophilic protein) in multimolar salt solutions.- Studying membrane proteins in detergent solution by analytical ultracentrifugation: different methods for density matching.- Analytical ultracentrifugation of the nitrogenase of Azotobacter vinelandii under anaerobic conditions.- Combining sedimentation velocity with SEC-MALLS to probe the molecular structure of heterogeneous macromolecular systems that cannot be preparatively separated: application to three rice starches.- Inhomogeneity and conformational parameters of low-substituted carboxymethyl cellulose from analytical ultracentrifugation data.- UV tagging leaves the structural integrity of an arabino-(4-O-methylglucurono)xylan polysaccharide unaffected.- Elevated-temperature analytical ultracentrifugation of a low-methoxy polyuronide.- Interacting systems and assemblies.- Towards deconvoluting the interaction of the Bacillus subtilis sporulation proteins SinR and SinI using tryptophan analogue incorporated proteins.- Use of the tryptophan analogue 7-azatryptophan to study the interaction of ? N with Escherichia coli RNA polymerase core enzyme.- Nonequilibrium self-association of a cpn60 chaperonin induced by tryptophan mutation.
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