This detailed book builds on the foundation of lymphatic protocols with tools to aid in the interpretation of large datasets created by angiogenesis research. For example, this includes protocols to explore how transcriptional phenotyping can be achieved, both in terms of exploring tissue-specific heterogeneity to the use of database mining of existing datasets. Similarly, bioinformatic techniques run alongside protocols for transcript analysis, cell biology, pathology, as well as physiological and developmental angiogenesis models. Written for the highly successful Methods in Molecular…mehr
This detailed book builds on the foundation of lymphatic protocols with tools to aid in the interpretation of large datasets created by angiogenesis research. For example, this includes protocols to explore how transcriptional phenotyping can be achieved, both in terms of exploring tissue-specific heterogeneity to the use of database mining of existing datasets. Similarly, bioinformatic techniques run alongside protocols for transcript analysis, cell biology, pathology, as well as physiological and developmental angiogenesis models. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Angiogenesis: Methods and Protocols provides a meaningful compendium of practical approaches to angiogenesis and endothelial cell biology research.
The Embryonic Mouse Hindbrain Model to Study Sprouting Angiogenesis In Vivo.- A Workflow to Track and Analyze Endothelial Migration during Vascular Development in Zebrafish Embryos Using Lightsheet Microscopy.- Analyzing Vessel Regression and Endothelial Apoptosis as a Component of Angiogenic Vessel Remodeling.- Whole-Mount Immunofluorescence Protocol for 3D Imaging, Reconstruction, and Quantification of 4th Pharyngeal Arch Artery Formation.- Quantifying and Characterizing Angiogenesis Using the Postnatal Mouse Retina.- Characterization of Lymphatic Vasculature Using Whole-Mount Immunostaining of Mouse Embryonic Dorsal Skin.- Analyzing Lymphatic Vessel Patterning in Adult Tissue.- Transmission Electron Microscopy of Endothelium.- Measurement of Revascularisation in the Hind Limb after Experimental Ischemia in Mice.- Imaging Blood Vessels and Lymphatics in Mouse Trachea Wholemounts.- Non-Invasive Measurement of Retinal Microvascular Permeability during Loss of Endothelial Quiescence.- An Ex Vivo Tissue Culture Method for Discovering Cell Dynamics Involved in Stromal Vascular Fraction Vasculogenesis Using the Mouse Mesentery.- Quantitative Three-Dimensional Analysis of the Lymphatic Vasculature in the Postnatal Mouse Heart.- Quantifying Lymphatic Vessel Density in Human Tissue Samples.- Quantifying Vascular Remodeling in the Mouse Spinal Cord.- Quantitative Methods to Assess Adipose Vasculature.- Quantification of Angiogenesis in Laser Choroidal Neovascularization.- A Rapid Adaptable Method for Isolation of Endothelial Cells from Human Adipose Tissue.- Simple Gene Knockdown in Endothelial Cells Using Short Interfering RNA Oligonucleotides.- Transcription Factor Chromatin Immunoprecipitation in Endothelial Cells.- In Vitro Co-Culture of Fibroblast and Endothelial Cells to Assess Angiogenesis.- Quantification of Leukocyte-Endothelial Cell Interaction during In Vitro Models of Thrombosis at Arterial and Venous Shear Rates.- Isolation of Cardiac Endothelial Cells forTranscriptomic Analysis of the Zebrafish and Mouse Heart.- The Evaluation of Neovessel Angiogenesis Behavior at Tissue Interfaces.- Endothelial Cell Fibrin Gel Angiogenesis Bead Assay.- Live Cell Imaging and Analysis of Cancer-Cell Transmigration through Endothelial Monolayers.- Differentiation and Characterization of Human Pluripotent Stem Cell-Derived Cardiac Endothelial Cells for In Vitro Applications.- Finding and Verifying Enhancers for Endothelial-Expressed Genes.- Endothelial Cell RNA-Seq Data: Differential Expression and Functional Enrichment Analyses to Study Phenotypic Switching.
The Embryonic Mouse Hindbrain Model to Study Sprouting Angiogenesis In Vivo.- A Workflow to Track and Analyze Endothelial Migration during Vascular Development in Zebrafish Embryos Using Lightsheet Microscopy.- Analyzing Vessel Regression and Endothelial Apoptosis as a Component of Angiogenic Vessel Remodeling.- Whole-Mount Immunofluorescence Protocol for 3D Imaging, Reconstruction, and Quantification of 4th Pharyngeal Arch Artery Formation.- Quantifying and Characterizing Angiogenesis Using the Postnatal Mouse Retina.- Characterization of Lymphatic Vasculature Using Whole-Mount Immunostaining of Mouse Embryonic Dorsal Skin.- Analyzing Lymphatic Vessel Patterning in Adult Tissue.- Transmission Electron Microscopy of Endothelium.- Measurement of Revascularisation in the Hind Limb after Experimental Ischemia in Mice.- Imaging Blood Vessels and Lymphatics in Mouse Trachea Wholemounts.- Non-Invasive Measurement of Retinal Microvascular Permeability during Loss of Endothelial Quiescence.- An Ex Vivo Tissue Culture Method for Discovering Cell Dynamics Involved in Stromal Vascular Fraction Vasculogenesis Using the Mouse Mesentery.- Quantitative Three-Dimensional Analysis of the Lymphatic Vasculature in the Postnatal Mouse Heart.- Quantifying Lymphatic Vessel Density in Human Tissue Samples.- Quantifying Vascular Remodeling in the Mouse Spinal Cord.- Quantitative Methods to Assess Adipose Vasculature.- Quantification of Angiogenesis in Laser Choroidal Neovascularization.- A Rapid Adaptable Method for Isolation of Endothelial Cells from Human Adipose Tissue.- Simple Gene Knockdown in Endothelial Cells Using Short Interfering RNA Oligonucleotides.- Transcription Factor Chromatin Immunoprecipitation in Endothelial Cells.- In Vitro Co-Culture of Fibroblast and Endothelial Cells to Assess Angiogenesis.- Quantification of Leukocyte-Endothelial Cell Interaction during In Vitro Models of Thrombosis at Arterial and Venous Shear Rates.- Isolation of Cardiac Endothelial Cells forTranscriptomic Analysis of the Zebrafish and Mouse Heart.- The Evaluation of Neovessel Angiogenesis Behavior at Tissue Interfaces.- Endothelial Cell Fibrin Gel Angiogenesis Bead Assay.- Live Cell Imaging and Analysis of Cancer-Cell Transmigration through Endothelial Monolayers.- Differentiation and Characterization of Human Pluripotent Stem Cell-Derived Cardiac Endothelial Cells for In Vitro Applications.- Finding and Verifying Enhancers for Endothelial-Expressed Genes.- Endothelial Cell RNA-Seq Data: Differential Expression and Functional Enrichment Analyses to Study Phenotypic Switching.
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