Potohar plateau (Punjab) of Pakistan comes under Punjab province and western parts of Azad Jammu & Kashmir, thus representing an Oriental distribution of insect species with traces of Palearctic fauna. It is spread over an area of about 15,830 sq.km having a latitudinal range of 32º 30' 34 º N and longitudinal range of 71 º 30' -74 º E The study was carried out to explore ant fauna of this important region of Pakistan. It comes up with a record of 21 species of ants with variable distribution pattern and habitat preferences.