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The present study was carried out in the Sena-Oura National Park (PNSO) located in the Mayo-Dallah department of Chad. The study was carried out between March and July 2020. The PNSO, which is home to six (06) villages, is subject to anthropogenic pressures on its natural resources. This study focused on characterizing the various pressures caused by man and their influence on natural resources. The various human-induced pressures on the PNSO's natural resources and their causes were identified through questionnaires, interviews and observations carried out with 120 people in the 04 villages sampled. These data were validated by fieldwork. Seven (07) pressures were identified: agriculture 33%, livestock breeding 18%, poaching 21%, bush fires 2%, logging 16%, NTFPs 7% and gold panning 3%.