An antigen is a substance that prompts the generation of antibodies and can cause an immune response. The antigen retrieval (AR) technique is used worldwide and has resulted in a revolution in immunohistochemistry (IHC). Featuring contributors who are distinguished experts and researchers in the field, this book discusses several scientific approaches to the standardization of quantifiable IHC. It summarizes the key problems in the four fields of antigen retrieval and provides practical methods and protocols in AR-IHC. Clinical pathologists, molecular cell biologists, basic research…mehr
An antigen is a substance that prompts the generation of antibodies and can cause an immune response. The antigen retrieval (AR) technique is used worldwide and has resulted in a revolution in immunohistochemistry (IHC). Featuring contributors who are distinguished experts and researchers in the field, this book discusses several scientific approaches to the standardization of quantifiable IHC. It summarizes the key problems in the four fields of antigen retrieval and provides practical methods and protocols in AR-IHC. Clinical pathologists, molecular cell biologists, basic research scientists, technicians, and graduate students, will benefit from this fully up-to-date work.Hinweis: Dieser Artikel kann nur an eine deutsche Lieferadresse ausgeliefert werden.
Produktdetails
Produktdetails
Wiley Series in Biomedical Engineering and Multi-Disciplinary Integrated Systems Vol.1
Shan-Rong Shi, MD, is professor of clinical pathology at the Keck School of Medicine of the University of Southern California. He is the Associate Editor of The Journal of Histochemistry and cytochemistry, he is on the Editoral Board of Applied Immunohistochemistry and Molecular Morphology and is also on the Overseas Editorial Board of the Chinese Journal of Pathology. Dr. Shi is the ineventor of the antigen retrieval (AR) technique. Clive R. Taylor, MD, PhD, is Senior Associate Dean, Professor and Chairman of Pathology at the Keck School of Medicine. Dr. Taylor is Editor in Chief of Applied Immunohistochemistry and Molecular Morphology. Dr. Taylor is a world renowned expert in immunohistochemistry and clinical pathology.
Inhaltsangabe
Preface ix Contributors xv Part I Recent Advances in Antigen Retrieval Techniques and Its Application 1 1 Standardization of Antigen Retrieval Techniques Based on the Test Battery Approach 3 Shan-Rong Shi and Clive R. Taylor 2 Extended Application of Antigen Retrieval Technique in Immunohistochemistry and in Situ Hybridization 25 Shan-Rong Shi and Clive R. Taylor 3 Extraction of Dna/rna From Formalin-fixed, Paraffin-embedded Tissue Based on the Antigen Retrieval Principle 47 Shan-Rong Shi and Clive R. Taylor Part II Standardization of Immunohistochemistry 73 4 Key Issues and Strategies of Standardization For Quantifiable Immunohistochemistry 75 Shan-Rong Shi, Kevin A. Roth, and Clive R. Taylor 5 Standardization of Immunohistochemistry Based on Antigen Retrieval Technique 87 Shan-Rong Shi and Clive R. Taylor 6 Standard Reference Material: Cell Line Development and Use of Reference Cell Lines as Standards for External Quality Assurance of Her2 Ihc and Ish Testing 101 Bharat Jasani, Vicky Reid, Colin Tristram, Jeremy Walker, Paul Scorer, Michael Morgan, John Bartlett, Merdol Ibrahim, and Keith Miller 7 Peptides as Immunohistochemistry Controls 123 Steven A. Bogen and Seshi R. Sompuram 8 Standard Reference Material: Protein- Embedding Technique and Design of Bar Code 141 Shan-Rong Shi, Jiang Gu, and Clive R. Taylor 9 the Pros and Cons of Automation for Immunohistochemistry From the Prospective Of the Pathology Laboratory 151 David G. Hicks and Loralee McMahon 10 Image Analysis in Immunohistochemistry 165 Alton D. Floyd Part III Tissue/cell Sample Preparation 187 11 Tissue Cell Sample Preparation: Lessons From the Antigen Retrieval Technique 189 Shan-Rong Shi and Clive R. Taylor 12 Mechanisms of Action and Proper Use of Common Fixatives 195 Richard W. Dapson 13 Cell Sample Preparation for Clinical Cytopathology: Current Status and Future Development 219 Yan Shi and Patricia G. Wasserman 14 Design of a Tissue Surrogate to Examine Accuracy of Proteomic Analysis 235 Carol B. Fowler, Jeffrey T. Mason, and Timothy J. O'leary Part IV Molecular Mechanism of Antigen Retrieval Technique 251 15 Study of Formalin Fixation and Heat-induced Antigen Retrieval 253 Jeffrey T. Mason, Carol B. Fowler, and Timothy J. O'leary 16 a Linear Epitopes Model of Antigen Retrieval 287 Steven A. Bogen and Seshi R. Sompuram 17 Ph Or Ionic Strength of Antigen Retrieval Solution: a Potential Role for Refolding During Heat Treatment 303 Shuji Yamashita 18 Commentary: Future Directions 323 Timothy J. O'Leary, Carol B. Fowler, David L. Evers, Robert E. Cunningham, and Jeffrey T. Mason Part V Proteomic Analysis of Protein Extracted From Tissue/cells 333 19 Techniques of Protein Extraction From Ffpe Tissue/cells for Mass Spectrometry 335 Carol B. Fowler, Timothy J. O'Leary, and Jeffrey T. Mason 20 Application of Shotgun Proteomics to Formalin-fixed and Paraffin-embedded Tissues 347 Brian M. Balgley 21 Visualizing Protein Maps in Tissue 369 Masahiro Mukai and Mitsutoshi Setou 22 Symbiosis of Immunohistochemistry and Proteomics: Marching to a New Era 391 Shan-Rong Shi, Brian M. Balgley, and Clive R. Taylor Appendix Related Laboratory Protocols 399 Index 413
Preface ix Contributors xv Part I Recent Advances in Antigen Retrieval Techniques and Its Application 1 1 Standardization of Antigen Retrieval Techniques Based on the Test Battery Approach 3 Shan-Rong Shi and Clive R. Taylor 2 Extended Application of Antigen Retrieval Technique in Immunohistochemistry and in Situ Hybridization 25 Shan-Rong Shi and Clive R. Taylor 3 Extraction of Dna/rna From Formalin-fixed, Paraffin-embedded Tissue Based on the Antigen Retrieval Principle 47 Shan-Rong Shi and Clive R. Taylor Part II Standardization of Immunohistochemistry 73 4 Key Issues and Strategies of Standardization For Quantifiable Immunohistochemistry 75 Shan-Rong Shi, Kevin A. Roth, and Clive R. Taylor 5 Standardization of Immunohistochemistry Based on Antigen Retrieval Technique 87 Shan-Rong Shi and Clive R. Taylor 6 Standard Reference Material: Cell Line Development and Use of Reference Cell Lines as Standards for External Quality Assurance of Her2 Ihc and Ish Testing 101 Bharat Jasani, Vicky Reid, Colin Tristram, Jeremy Walker, Paul Scorer, Michael Morgan, John Bartlett, Merdol Ibrahim, and Keith Miller 7 Peptides as Immunohistochemistry Controls 123 Steven A. Bogen and Seshi R. Sompuram 8 Standard Reference Material: Protein- Embedding Technique and Design of Bar Code 141 Shan-Rong Shi, Jiang Gu, and Clive R. Taylor 9 the Pros and Cons of Automation for Immunohistochemistry From the Prospective Of the Pathology Laboratory 151 David G. Hicks and Loralee McMahon 10 Image Analysis in Immunohistochemistry 165 Alton D. Floyd Part III Tissue/cell Sample Preparation 187 11 Tissue Cell Sample Preparation: Lessons From the Antigen Retrieval Technique 189 Shan-Rong Shi and Clive R. Taylor 12 Mechanisms of Action and Proper Use of Common Fixatives 195 Richard W. Dapson 13 Cell Sample Preparation for Clinical Cytopathology: Current Status and Future Development 219 Yan Shi and Patricia G. Wasserman 14 Design of a Tissue Surrogate to Examine Accuracy of Proteomic Analysis 235 Carol B. Fowler, Jeffrey T. Mason, and Timothy J. O'leary Part IV Molecular Mechanism of Antigen Retrieval Technique 251 15 Study of Formalin Fixation and Heat-induced Antigen Retrieval 253 Jeffrey T. Mason, Carol B. Fowler, and Timothy J. O'leary 16 a Linear Epitopes Model of Antigen Retrieval 287 Steven A. Bogen and Seshi R. Sompuram 17 Ph Or Ionic Strength of Antigen Retrieval Solution: a Potential Role for Refolding During Heat Treatment 303 Shuji Yamashita 18 Commentary: Future Directions 323 Timothy J. O'Leary, Carol B. Fowler, David L. Evers, Robert E. Cunningham, and Jeffrey T. Mason Part V Proteomic Analysis of Protein Extracted From Tissue/cells 333 19 Techniques of Protein Extraction From Ffpe Tissue/cells for Mass Spectrometry 335 Carol B. Fowler, Timothy J. O'Leary, and Jeffrey T. Mason 20 Application of Shotgun Proteomics to Formalin-fixed and Paraffin-embedded Tissues 347 Brian M. Balgley 21 Visualizing Protein Maps in Tissue 369 Masahiro Mukai and Mitsutoshi Setou 22 Symbiosis of Immunohistochemistry and Proteomics: Marching to a New Era 391 Shan-Rong Shi, Brian M. Balgley, and Clive R. Taylor Appendix Related Laboratory Protocols 399 Index 413
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