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The aim of this in vitro study was to compare the antimicrobial efficacy of 2%Propolis, 6%HEBP , 17%EDTA and 2 % Chlorhexidine against Enterococcus Faecalis and Candida Albicans .The method employed for this procedure was the Agar Cup Method ,using Sterile Brain Heart Infusion Agar for Enterococcus Faecalis and Sterile Sabourauds Agar for Candida Albicans.After the Incubation period, the mean zones of inhibition were observed and noted the standard mean deviation was derived for samples containing Enterococcus Faecalis and Candida Albicans . For samples containing Enterococcus Faecalis ,the standard mean for Propolis , HEBP, Chlorhexidine and EDTA was 15.33mm,23.00mm,19.66mm and 21.00mm respectively . For samples containing Candida Albicans ,the standard mean for Propolis, HEBP, Chlorhexidine and EDTA was 13.33mm,20.33mm,22.00mm,and19.33mm respectively . It was observed that though Propolis showed a good antimicrobial activity the highest was shown by HEBP.This study indicates that while Propolis may not be as promising as previously believed, HEBP has good potential to be commercially viable as a root canal irrigant if it shows good performance in invivo clinical trials .