An agar well diffusion assay method was used to determine the efficacy of the root canal sealer against E. faecalis (ATCC 29212). Root canal sealers were divided into three groups; BC sealer, Epiphany self-etch sealer, and AH-Plus sealer, and standard antibiotic disc of co-trimoxazole was kept as a control. The diameters of the growth inhibition zones against E. faecalis for each group were recorded and compared at 24 h and 48 h. The differences between groups were analyzed by one-way ANOVA and Tukey's post hoc tests for intergroup analysis.