Subclinical mastitis in buffaloes is a silent yet costly threat to the dairy industry, often going undetected while impacting milk production and animal health. This study investigates the antimicrobial efficacy of Psidium guajava (guava) leaf extracts in treating subclinical mastitis in buffaloes. Subclinical mastitis, an infection of the mammary glands without visible symptoms, is commonly caused by bacteria such as Escherichia coli and Staphylococcus aureus. The research aims to evaluate the effectiveness of guava leaf extracts as a natural alternative to antibiotics, addressing antimicrobial resistance issues. The findings may highlight the potential of guava leaves as a cost-effective, plant-based treatment for improving buffalo health and milk production.