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Plants can be used as therapeutic agent is an ancient thought. The use of medicinal plants as a source for relief from illness can be traced from early civilization in India. Therefore, the present study is aimed at investigating five plants namely, Aconitum napellus, Cassia tora, Embelia ribes, Alpinia galanga and Jujuba for their phytochemical screening and antimicrobial efficacy to prove to ethnomedicinal uses. Authors convey deep thanks to our great, young and dynamic Directors Shri Arpit Agarwal ji and Shri Amit Agarwal ji for unconditional support, family feelings, and everlasting encouragement.Authors feel immense pleasure to give thanks from bottom of our heart to our Academic Director Prof. C. K. Ojha for become a guiding force for our research work. Authors are also grateful to Department of Science and Technology, Jaipur for providing us financial support under the auspcies of Centre with Potential for Excellence in Biotechnology