Antimicrobial drugs have caused a dramatic change not only for the treatment of infectious diseases but also as a fate of mankind. Contrary to the synthetic drugs, antimicrobials of plant origin are not associated with side effects and have an enormous therapeutic potential to heal many infectious diseases. The present manual provides valuable information regarding Antimicrobial Activity of Jasminum multiflorum (Burm. f.) Andrews, Stephania glabra (Roxb.) Miers & Cirsium cymosum (Greene) J. T. Howell. This manuscript has been illustrated with 11 tables, 10 figures and more than 62 coloured photographs, clearly representing the usefulness of the treatise. The present manual will attract the attention of Pharmacologists, Phytochemists, Policy planners, and students, researchers, teachers as well as scientists in the field of Microbiology, Ethnobotany and Biotechnology.