The present study was design to determine in-vitro antioxidant potential and fracture healing activity of Lepidium sativum seeds extract. This book consists of phytochemical screening, determine of the total Phenolic and Flavonoid content, antioxidant activity, acute toxicity study and Fracture healing activity. The phytochemical studies of extract has been revealed that plant extract consists of various amino acids, like glutamine, cysteine, and glycine and Phenolic and Flavonoids content which are produced good antioxidant potential. The glycine may be most prominent participation in fracture healing activity and its acts as main precursor of collagen synthesis. The Collagen I type approximately 90 % total bone protein.