Marema Makgatho

Antiplasmodial activties of TMP-phenazines B4158 and B4119

• Broschiertes Buch

Produktbeschreibung

A novel flow cytometric procedure was established for use in evaluating the in vitro antimalarial activity oftetramethylpiperidine (TMP)- substituted phenazines. The flow cytometric procedure was compared with microscopy and radiometry for efficiency in quantitating the level of parasitemia in malaria cultures. The flow cytometric method compared well, as determined by the Bland and Altman measure of agreement, with both microscopy and radiometry and was chosen for use in this study due to its speed, precision and convenience (includes a fixing step that allows samples to be evaluated at anyone time). The TMP-substituted phenazines B4119 and B4158, synthetic derivatives of clofazimine, were evaluated extensively against a drug-sensitive and various drug-resistant lines ofPlasmodium jalciparum in vitro and against P. berghei in mice. Parasite growth was measured using microscopic and flow cytometric methods, while heme polymerization was investigated using an infrared spectroscopic procedure. The therapeutic potential of B4119 alone (30mg/kg/day), and in combination with a sub-therapeutic dose of chloroquine (l.251lg/kg/day) was measured in a murine model of experimental infection.

Produktdetails

• Verlag: LAP Lambert Academic Publishing
• Seitenzahl: 184
• Erscheinungstermin: 18. April 2019
• Englisch
• Abmessung: 220mm x 150mm x 12mm
• Gewicht: 292g
• ISBN-13: 9783330062535
• ISBN-10: 3330062533
• Artikelnr.: 56610535

Autorenporträt

Le Dr Marema Ephraim Makgatho a obtenu une licence en sciences médicales à l'Université du Nord en 1993, avec une spécialisation en immunologie. Il a poursuivi ses études de troisième cycle à l'université de Pretoria et a obtenu un doctorat en 2000. Depuis 1999, il est chargé de cours en immunologie et microbiologie médicale à l'université de Limpopo.