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In this book, we have established a green and highly efficient one-pot three component microwave irradiation protocol for the synthesis of various quinoline-4-carboxylic acidscatalyzed by p-TSA. The p-TSA catalyst was easily commercially available and non-hazardous. There are many features of this protocol including high yield of products with high purity as well as lower reaction time compared to conventional methods, a simple work-up processand avoidance of the use of hazardous organic solvents.A simple work-up procedure make the present method a valuable contribution in agreement with green chemistry principles. We have synthesized compounds 4a-4p and tested their biological activity. Molecular docking study was suggested that synthesized compounds were fit into the active site of protein and predict best mechanism of in-silico side. Best dock compound 4c was selected for the molecular dynamics.