In last two decades, bioinformatics plays a very significant role in modern scientific research. It is forming the backbone of biotechnology through the systematic storage and manipulation of flood of information produced during biological experiments, which solve and short the life science concerns. In this interdisciplinary area, researchers use mathematical, statistical and computational skills in order to develop advanced technologies for easy handling of biological data. Moreover, research on fisheries and aquaculture has been growing expeditiously in order to meet the future food demand pinpointing the frequent need of bioinformatics knowledge towards achieving the innovative outputs in a smooth approach. At the same time, researchers having poor insight in bioinformatics tools and techniques could lead to missed opportunities or misperception of information. In this paper, the focus is to delineate some key concepts about the familiar tools, databases, and importance of bioinformatics along with their application in fishery science.