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Conventional plants are precious source of novel cytotoxic agents and are still in performance better role in health concern. The study was intended to estimation of the anticancer activity of Methanolic Extract of Aristolochia indica on human chronic mylogenous leukemia cancer cell lines. Extract was tested for its inhibitory effect in 96 micro plate formats against K562 Cell Line. Plant gave good apoptotic DNA fragmentation. Summaries the data of the activities on in-vitro screening of Aristolochia indica plant extract, its gave positive activity on Cytotoxicity and Antimicrobial activity. The plant also gives Antimutagenic activity concluded from mutation study.