Aspects of differentiated stem cells for in vivo reconstitution - Achatz-Straussberger, Gertrude;Schwaiger, Elisabeth
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It was our aim to establish an in vitro differentiation system to prime BALB/c IgM-/-embryonic stem cells (ESCs) for lymphocyte development. We characterised the BALB/c IgM-/- ESC line for markers of pluripotency by fluorescence-activated cell sorting (FACS) and by epifluorescence. Thus, we characterised this cell line for the congenic marker expression of CD45.1 and CD45.2 on their surface. As B cell development takes place in the bone marrow, the differentiated embryonic stem cells should have the capacity to home to the developmental niches after transfer. Therefore, we investigated the…mehr

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Produktbeschreibung
It was our aim to establish an in vitro differentiation system to prime BALB/c IgM-/-embryonic stem cells (ESCs) for lymphocyte development. We characterised the BALB/c IgM-/- ESC line for markers of pluripotency by fluorescence-activated cell sorting (FACS) and by epifluorescence. Thus, we characterised this cell line for the congenic marker expression of CD45.1 and CD45.2 on their surface. As B cell development takes place in the bone marrow, the differentiated embryonic stem cells should have the capacity to home to the developmental niches after transfer. Therefore, we investigated the homing capacity through Transwell migration assays against C-X-C motif chemokine 12 (CXCL12=SDF1), which represents a powerful attractant for stem cells and progenitor cells and guides the cells to their developmental bone marrow niches.
Autorenporträt
Achatz-Straussberger G. completed masters and doctoral degrees in science (University of Vienna). After postdoc position at the Med Science Society, at the Dep of Genetics (University of Salzburg) and the Hertha Firnberg Fellowship she reached the position of a Senior Scientist at the Dep of Molecular Biology at the University of Salzburg.