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In order to evaluate genetic diversity cultivated sainfoin accessions collected from different regions of the world, SSR markers from medicago genus were used . polymorphism information content value ranged from 0.2 to .043 with an average of 0.33. cluster analysis based on SSR markers grouped the sainfoin population in two main group (Iranian and exotic cultivate sainfoin) with two subcluster foreach group.An other survey is efficient of echological factors on genetic similarity was investigated among five population of onobrychis viccifolia East Azerbaijan , Iran using RAPD and bulked DNA, The largest and shortest genetic were detected between Bonab and Heris (0.04904) and Amand and khosroshahr(0.0572) on UPGMA dendrogram, two population of sarab and Heris were nested within one cluster, and Amand and khosroshahr in other cluster. Also Bonab was completely separated from all other population . Also the other survey is SRAP marker that couldn't justify genetic diversity in saifoin completely.