Global monitoring indicates that only 39% of all infants worldwide are exclusively breastfed. Therefore, the study seeks to assess knowledge, attitude and practices of Exclusive Breast Feeding (EBF) among working nursing mothers in the Sekyere South District of the Ashanti Region. A descriptive cross-sectional study design was used. The study population considered working nursing mothers who were attending welfare clinic with a baby for immunization and growth monitoring and whose last child was between six to twelve months. A simple random sampling method was used to choose two health facilities from each of the five sub-districts from where the sample was selected