With the number of cases of chronic hepatitis C on the rise, these infections have become a cause for concern. Objectives: Evaluate and enrich nurses' knowledge about HCV and help improve the management of patients with chronic hepatitis C. Materials and methods: Descriptive study directed at nurses working at Gabès hospital. The survey took place in February and March 2023.results: 80 nurses were included, with a sex ratio of 0.56. Half of the population (52%) had no previous training in hepatitis C. The majority of nurses mentioned that the causative agent was a virus. Regarding preventive practices, the majority of nurses respected hand washing (86%), wearing gloves (82%) and masks (66%), and not recapping needles (86%). However, there was a lack of knowledge of treatment (62%), which confirms that nurses are unfamiliar with certain details that require awareness-raising and training to improve management.