Thissecond edition expands upon the previous volume with new and updated chapters. Auditory and Vestibular Research: Methodsand Protocols, Second Edition guides readers through protocols on cellculture, tissue engineering, nanotechnology, high-throughput screening, andphysiology. Chapters on physiology covertechniques that include optical coherence tomography, patch clamping, andphotostimulation of caged neurotransmitters. Written in the highly successful Methods in Molecular Biology seriesformat, chapters include introductions to their respective topics, lists of thenecessary materials and…mehr
Thissecond edition expands upon the previous volume with new and updated chapters. Auditory and Vestibular Research: Methodsand Protocols, Second Edition guides readers through protocols on cellculture, tissue engineering, nanotechnology, high-throughput screening, andphysiology. Chapters on physiology covertechniques that include optical coherence tomography, patch clamping, andphotostimulation of caged neurotransmitters. Written in the highly successful Methods in Molecular Biology seriesformat, chapters include introductions to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritativeand cutting-edge, Auditoryand Vestibular Research: Methods and Protocols, Second Edition aims to ensure successfulresults in the further study of this vital field.
Helios®Gene Gun-Mediated Transfection of the Inner Ear Sensory Epithelium: RecentUpdates.- Tol2-MediatedDelivery of miRNAs to the Chicken Otocyst using Plasmid Electroporation.- A Rapid,Cost-Effective Method of Recombinant Adeno-Associated Virus Preparation EnablesEfficient Gene Transfer to the Developing Mouse Inner Ear.- Generationof Noninvasive, Quantifiable, Orthotopic Animal Models for NF2-Associated Schwannomaand Meningioma.- RNAExtraction from Xenopus Auditory and Vestibular Organs for Molecular Cloningand Expression Profiling with RNA-Seq and Microarrays .- YeastTwo-Hybrid Screening to Test for Protein-Protein Interactions in the AuditorySystem.- MultiplexedIsobaric Tagging Protocols for Quantitative Mass Spectrometry Approaches toAuditory Research.- ProteinQuantification of the Developing Cochlea by Mass Spectrometry.- UltrastructuralIdentification and Colocalization of Interacting Proteins in the Murine Cochleaby Post-Embedding Immunogold Transmission Electron Microscopy.- Surface Plasmon Resonance (SPR) Analysis ofBinding Interactions of Inner-Ear Proteins.- The Single-Molecule Approach to MembraneProtein Stoichiometry.- Visualization of Live Cochlear Stereocilia ata Nanoscale Resolution Using Hopping Probe Ion Conductance Microscopy.- Design and Construction of a Cost-EffectiveSpinning Disk System for Live Imaging of Inner Ear Tissue.- Neuroanatomical Tracing Techniques: History,State of the Art, and Future Developments.- Detection of Excitation and Inhibition in theAuditory System Using Fluorescence Immunohistochemistry and High ResolutionFluorescence Microscopy.- Rapid and Semi-Automated Extraction ofNeuronal Cell Bodies and Nuclei from Electron Microscopy Image Stacks.-Organotypic Culture of the Mouse Cochlea fromEmbryonic Day 12 to the Neonate.- Quantifying Spiral Ganglion Neurite andSchwann Behavior on Micropatterned Polymer Substrates.- The Use of Human Wharton's Jelly Cells forCochlear Tissue Engineering.- Nanotechnology in Auditory Research: MembraneElectromechanics in Hearing.- Overview of Nanoparticle Based Delivery for Treatment of Inner Ear Disorders.- Development of Cell-based High-Throughput ChemicalScreens for Protection Against Cisplatin-induced Ototoxicity.-Profiling Specific Inner Ear Cell Types UsingCell Sorting Techniques.- Optical Coherence Tomography to MeasureSound-Induced Motions Within the Mouse Organ of Corti In Vivo.- Method for Dissecting the Auditory Epithelium(Basilar Papilla) in Developing Chick Embryos.- Whole Cell Patch Clamp Recording of Mouse andRat Inner Hair Cells in the Intact Organ of Corti.- Glass Probe Stimulation of Hair CellStereocilia.- A Walkthrough of Nonlinear CapacitanceMeasurement of Outer Hair Cells.- In Vitro Functional Assessment of AdultSpiral Ganglion Neurons (SGNs).- Mapping Auditory Circuits withPhotostimulation of Caged Glutamate.
Helios®Gene Gun-Mediated Transfection of the Inner Ear Sensory Epithelium: RecentUpdates.- Tol2-MediatedDelivery of miRNAs to the Chicken Otocyst using Plasmid Electroporation.- A Rapid,Cost-Effective Method of Recombinant Adeno-Associated Virus Preparation EnablesEfficient Gene Transfer to the Developing Mouse Inner Ear.- Generationof Noninvasive, Quantifiable, Orthotopic Animal Models for NF2-Associated Schwannomaand Meningioma.- RNAExtraction from Xenopus Auditory and Vestibular Organs for Molecular Cloningand Expression Profiling with RNA-Seq and Microarrays .- YeastTwo-Hybrid Screening to Test for Protein-Protein Interactions in the AuditorySystem.- MultiplexedIsobaric Tagging Protocols for Quantitative Mass Spectrometry Approaches toAuditory Research.- ProteinQuantification of the Developing Cochlea by Mass Spectrometry.- UltrastructuralIdentification and Colocalization of Interacting Proteins in the Murine Cochleaby Post-Embedding Immunogold Transmission Electron Microscopy.- Surface Plasmon Resonance (SPR) Analysis ofBinding Interactions of Inner-Ear Proteins.- The Single-Molecule Approach to MembraneProtein Stoichiometry.- Visualization of Live Cochlear Stereocilia ata Nanoscale Resolution Using Hopping Probe Ion Conductance Microscopy.- Design and Construction of a Cost-EffectiveSpinning Disk System for Live Imaging of Inner Ear Tissue.- Neuroanatomical Tracing Techniques: History,State of the Art, and Future Developments.- Detection of Excitation and Inhibition in theAuditory System Using Fluorescence Immunohistochemistry and High ResolutionFluorescence Microscopy.- Rapid and Semi-Automated Extraction ofNeuronal Cell Bodies and Nuclei from Electron Microscopy Image Stacks.-Organotypic Culture of the Mouse Cochlea fromEmbryonic Day 12 to the Neonate.- Quantifying Spiral Ganglion Neurite andSchwann Behavior on Micropatterned Polymer Substrates.- The Use of Human Wharton's Jelly Cells forCochlear Tissue Engineering.- Nanotechnology in Auditory Research: MembraneElectromechanics in Hearing.- Overview of Nanoparticle Based Delivery for Treatment of Inner Ear Disorders.- Development of Cell-based High-Throughput ChemicalScreens for Protection Against Cisplatin-induced Ototoxicity.-Profiling Specific Inner Ear Cell Types UsingCell Sorting Techniques.- Optical Coherence Tomography to MeasureSound-Induced Motions Within the Mouse Organ of Corti In Vivo.- Method for Dissecting the Auditory Epithelium(Basilar Papilla) in Developing Chick Embryos.- Whole Cell Patch Clamp Recording of Mouse andRat Inner Hair Cells in the Intact Organ of Corti.- Glass Probe Stimulation of Hair CellStereocilia.- A Walkthrough of Nonlinear CapacitanceMeasurement of Outer Hair Cells.- In Vitro Functional Assessment of AdultSpiral Ganglion Neurons (SGNs).- Mapping Auditory Circuits withPhotostimulation of Caged Glutamate.
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