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Auxins are a group of plant hormones and growth regulators that are synthetized naturally by plants or manufactured artificially, and that play a major role in plant micropropagation. The use of auxins influence cell division, growth, differentiation and in vitro morphogenesis. Thus, auxins have been widely used to induce rhizogenesis, caulogenesis, callogenesis and somatic embryogenesis. Auxins are also known for their role in maintaining apical dominance and plant polarity, and are involved in the plant signaling systems, which allows plant cells to interact among them in response to different exogenous factors and culture conditions. The present book presents the different natural and synthetic auxins, describes their roles and modes of action and reports results from literature that highlight their significant impacts in plant micropropagation.