Educational level, age, monthly income, total family size, reading habit and use of media had shown statistically significant association with response variable (awareness level) for both Adama and Assela cities. Only about 45.8% and 44.9% of the respondents at Adama and Assela cities respectively are aware of the infectious disease while the rest are not aware. Although 33.5% and 33.3% of the respondents on Adama and Assela cities respectively were aware of the availability of vaccine for Hepatitis B, only 6.6% and 6.8% of the respondents are vaccinated for Hepatitis B.