This study aims to evaluate the chemical profile and fungicidal activity of the nanoemulsion of the essential oil obtained from Ba-har (Syrian pepper). The results obtained for the total phenolic content were quantified in 348.3 mg EAT g-1 and 346.21 mg EQ g-1 for flavonoids. Ba-har essential oil was more efficient in inhibiting A niger, as it presented the lowest MIC (200 µg mL-1), followed by P. chrysogenum (250 µg mL-1) and later by C. gloeosporioides (300 µg mL-1). The identification of secondary metabolites present in Ba-har was quite considerable, since they are responsible for biological properties, thus, inspiring the continuity of studies related to its biological activities. As for the total phenolic content and flavonoids present in the essential oil, they indicated the important antioxidant potential. The fungicidal potential of Ba-har oil showed strong inhibition and mortality, however the nanoemulsion product with the essential oil incorporated showed a more efficient action against the pathogenic fungi tested.