This handbook is a concise synopsis of practical aspects of Veterinary Bacteriology and Mycology. It summarizes principles and procedures of most laboratory diagnostic tests performed in the microbiology laboratory. The focus is mainly on the general Bacteriology and Mycology laboratory techniques usually employed in the isolation and identification of animal pathogens. The handbook also covers the main characteristics and tests employed in the identification of specific genera and species of both bacteria and fungi associated with animal infections. It is a good handbook not only for veterinary undergraduate students, and microbiology laboratory diagnosticians but also for students of biomedical laboratory sciences.