The use of animal, including human, cell culture has expanded enormously during the last twenty years, with the new applications appearing all the time. This book guides the newcomer progressively through all those areas which are basic to the performance of cell culture. It will also prove useful to the experienced worker entering a new field or setting up a new laboratroy, and as a source of reference on basic techniques. The topics covered include setting up and equipping a cell culture laboratory, sterilization of fluids and equipment, culture media, basic culture technique and the…mehr
The use of animal, including human, cell culture has expanded enormously during the last twenty years, with the new applications appearing all the time. This book guides the newcomer progressively through all those areas which are basic to the performance of cell culture. It will also prove useful to the experienced worker entering a new field or setting up a new laboratroy, and as a source of reference on basic techniques. The topics covered include setting up and equipping a cell culture laboratory, sterilization of fluids and equipment, culture media, basic culture technique and the maintenance of cell lines, primary culture and the isolation of new cell lines, specific cell types and their requirements, single cell cloning, quality control of cell lines and the prevention, detection, and cure of contamination.
About the Author: A retired Ph.D. nurse, specializing in mental health, Shoni Davis lives in Boise, Idaho. She devoted much of her forty-year nursing career to working with women experiencing mental health and addiction issues. She learned as much from them about life as she hoped they learned from her. Shoni is the mother of two grown daughters and five awesome grandkids. Quantum physics intrigues her. Don't ask her to repeat back what she just read-but the part about everything being possible she finds fascinating. Esme is Shoni's debut novel.
Inhaltsangabe
* 1: Caroline Wigley: The Cell Culture Laboratory * 2: Peter Roberts: Sterilization * 3: T Cartwright and G P Shah: Culture Media * 4: Ian Dobbie and Daniel Zicha: Microscopy of Living Cells * 5: James McAteer and John M Davis: Basic cell culture and the maintenance of cell lines * 6: Caroline McDonald: Primary culture and the establishment of cell lines * 7: J D Sato et al: Specific cell types and their requirements * 8: John Clarke, Alison Porter, Robin Thorpe, and John M Davis: Cloning * 9: B J Bolton, P Packer and A Doyle: The quality control of cell lines * 10: Barbara Clitherow: Good laboratory practice in the cell culture laboratory * Appendix
* 1: Caroline Wigley: The Cell Culture Laboratory * 2: Peter Roberts: Sterilization * 3: T Cartwright and G P Shah: Culture Media * 4: Ian Dobbie and Daniel Zicha: Microscopy of Living Cells * 5: James McAteer and John M Davis: Basic cell culture and the maintenance of cell lines * 6: Caroline McDonald: Primary culture and the establishment of cell lines * 7: J D Sato et al: Specific cell types and their requirements * 8: John Clarke, Alison Porter, Robin Thorpe, and John M Davis: Cloning * 9: B J Bolton, P Packer and A Doyle: The quality control of cell lines * 10: Barbara Clitherow: Good laboratory practice in the cell culture laboratory * Appendix
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