Basic Cloning Procedures

Herausgegeben:Berzins, Valdis

• Broschiertes Buch

Produktbeschreibung

This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis. Each protocol includes short background information, a detailed description of the necessary materials, step-by-step procedures, a troubleshooting guide and useful practical hints.

Produktdetails

• Springer Lab Manual
• Verlag: Springer / Springer Berlin Heidelberg / Springer, Berlin
• Artikelnr. des Verlages: 978-3-642-48977-8
• 1998
• Seitenzahl: 180
• Erscheinungstermin: 7. Juli 2012
• Englisch
• Abmessung: 235mm x 155mm x 11mm
• Gewicht: 284g
• ISBN-13: 9783642489778
• ISBN-10: 364248977X
• Artikelnr.: 39157931

Inhaltsangabe

1 cDNA Synthesis and Cloning.- 2 DNA Sequencing.- 3 RNA In Vitro Synthesis by Phage T7 DNA-Dependent RNA Polymerase.- 4 PCR-Based Site-Specific Mutagenesis.- 5 Analysis of Specific Protein-DNA Interactions.- 6 Gel Electrophoresis, Transfer and Hybridization of Nucleic Acids.- 7 Immunological Methods for Analysis of Recombinant Proteins.- 8 Protein Synthesis in Cell-Free Systems.