Basil Proteomics - Sharma, Vikas;Singh Jadon, Vikash
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As a part of the systematic and proteomic approach based characterization of plant material with the advantage of producing valuable informations of functional genomics for a general crop improvement programme, leaves of holy medicinal-aromatic plant Tulsi (Ocimum basilicum L.) were used as a source of proteins and a model eukaryotic system. In this study, we developed a reliable protocol for PEG mediated enrichment of low abundant cellular proteins to increase their detection through 2 DE-SDS PAGE. I6% PEG was found to be optimum for the precipitation of Ocimum protein. Further, proteome…mehr

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Produktbeschreibung
As a part of the systematic and proteomic approach based characterization of plant material with the advantage of producing valuable informations of functional genomics for a general crop improvement programme, leaves of holy medicinal-aromatic plant Tulsi (Ocimum basilicum L.) were used as a source of proteins and a model eukaryotic system. In this study, we developed a reliable protocol for PEG mediated enrichment of low abundant cellular proteins to increase their detection through 2 DE-SDS PAGE. I6% PEG was found to be optimum for the precipitation of Ocimum protein. Further, proteome proteins in different fractions were analysed electrophoretically including 2- DE technique. These protocols may also will helpful in further studies in basil proteomics and other plants also.
Autorenporträt
Vikas Sharma has done his MSc. and Ph.D in Biotechnology From SBSPGI, Balawala (Dehradun)/ HNB Garhwal Central University, Srinagar, India. He has qualified reputed GATE-BT. Presently he is Asst. Professor, Biotechnology in Arni University,Kathgarh, Himachal Pradash (India). His current area of research is Plant microbe interaction and Biotization.