Biobanking

Methods and Protocols
Herausgegeben:Yong, William H.

• Gebundenes Buch

Produktbeschreibung

This volume not only discusses various common biobanking topics, it also delves into less-discussed subjects such as what is needed to start a biobank, training of new biobanking personnel, and ethnic representation in biospecimen research. Other chapters in this book span practical topics including: disaster prevention and recovery; information technology; flora and fauna preservation including zoological fluid specimen photography; surgical and autopsy biobanking; biobanking of bodily fluids; biosafety; cutting frozen sections; immunohistochemistry; nucleic acid extraction; and biospecimen shipping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Unique and comprehensive, Biobanking: Methods andProtocols is a valuable resourcefor novice and practicing biobankers, and for end-user researchers. This book aims to bring new insight into the field and expand on current biomedical biobanking studies.

Produktdetails

• Methods in Molecular Biology 1897
• Verlag: Humana / Springer New York / Springer, Berlin
• Artikelnr. des Verlages: 978-1-4939-8933-1
• 1st ed. 2019
• Seitenzahl: 464
• Erscheinungstermin: 12. Dezember 2018
• Englisch
• Abmessung: 260mm x 183mm x 31mm
• Gewicht: 1028g
• ISBN-13: 9781493989331
• ISBN-10: 1493989332
• Artikelnr.: 53513725

Inhaltsangabe

Sustainability in Biobanking- An Introduction to Starting a Biobank.- An Introduction to Hardware, Software, and Other Information Technology Needs of Biomedical Biobanks.- Disaster Prevention and Recovery.- Minority Participation in Biobanks: An Essential Key to Progress.- Orientation and Training of New Biobank Personnel.- Procurement and Storage of Surgical Biospecimens.- Autopsy Biobanking: Biospecimen Procurement, Integrity, Storage, and Utilization.- Procurement, Storage, and Use of Blood in Biobanks.- Procurement, Transportation, and Storage of Saliva, Buccal Swab, and Oral Wash Specimens.- Biobanking of Cerebrospinal Fluid.- Biobanking of Urine Samples.- Procurement and Storage of Pleural and Peritoneal Fluids for Biobanking.- The Fluid Preservation of Zoological Specimens.- Photographing Fluid Preserved Specimens.- Casts of Fluid Preserved Specimens.- Collection and Preservation of Terrestrial Arthropods.- Procurement and Preservation of Plants.- Biosafety and Biohazards: Understanding Biosafety Levels and Meeting Safety Requirements of a Biobank.- Decontamination of Biobank Facilities.- Laboratory Safety: Chemical and Physical Hazards.- Making Formalin-Fixed Paraffin Embedded Blocks.- Microtomy: Cutting Formalin-Fixed Paraffin Embedded Sections.- Performing and Cutting Frozen Sections.- An Introduction to the Performance of Immunohistochemistry.- An Introduction to Performing Immunofluorescence Staining.- Making a Tissue Microarray.- Nucleic Acid Isolation and Quality Control.- Fundamentals of RNA Analysis on Biobanked Specimens.- Nucleic Acid Extraction from Human Biological Samples.- An Overview of DNA Analytical Methods.- Shotgun Proteomic Profiling of Blood Borne Nanoscale Extracellular Vesicles (NsEVs).- Sample Preparation for Transmission Electron Microscopy.- Processing of Primary Patient Tumors and Subsequent Generation of Primary Cell Lines.- Domestic and International Shipping of Biospecimens.